ABSTRACT
Aim: A study was conducted to evaluate the salinity tolerance of white jute (C. capsularis) cultivars. Methodology: Five white jute cultivars were assessed for different salinity concentrations (0, 100, 150, 200 and 250 mM NaCl) in a split plot design with three replications per concentration under greenhouse condition. A total of fifteen plants were sampled from each treatment ten days after treatment with NaCl to determine morphological and physiological parameters. Results: Increased NaCl concentrations reduced all the morphological and physiological parameters such as plant height, root length, number of leaves, leaf area, shoot and root dry weight, relative leaf water content (RLWC), chlorophyll, protein, proline content, K+ accumulation in shoot and leaves, but water saturation deficit (WSD) and Na+ contents were increased in the shoot and leaves. Interpretation: The study revealed that among all cultivars assessed JRC-532 and JRC-321 showed relatively better performance against salt stress whereas cultivar JRC-517 was found more susceptible to salt stress.
Subject(s)
Adolescent , Adult , Anti-Bacterial Agents/pharmacology , Antibodies, Bacterial/blood , Bacteriological Techniques/methods , Child , Child, Preschool , Disease Outbreaks , Female , Humans , India/epidemiology , Infant , Latex Fixation Tests , Male , Meningococcal Infections/epidemiology , Meningococcal Infections/microbiology , Microbial Sensitivity Tests , Middle Aged , Nasopharynx/microbiology , Neisseria meningitidis/isolation & purification , Sex Distribution , Young AdultABSTRACT
A prospective study was undertaken to compare the Polymerase Chain Reaction (PCR) and Quantitative Buffy Coat (QBC) assay with conventional Giemsa technique for diagnosis of malaria. A total of 104 samples were taken for the purpose. They comprised of fever cases suggestive of malaria (n=74) and control group, fever cases other than malaria (n=30). Peripheral blood smears were prepared by Giemsa staining and QBC assay was performed as per standard protocol. From the stored blood samples, parasite DNA was extracted and PCR was performed using P. falciparum and P. vivax specific sets of primers. The QBC assay was 100% in agreement with the Giemsa stain. Specificity of the PCR detection of P. falciparum parasites was 100%. However, sensitivity for detection of P. falciparum and P. vivax by PCR was 64.28% and 82.35% respectively. In mixed cases of malaria (n=2), PCR results were in 100% agreement with that of Giemsa. The lower sensitivity of PCR for P. falciparum could probably be due to inaccessibility of target DNA, presence of PCR inhibitors in samples and parasite strain variations.